rabbit anti human parp1  (Proteintech)

Journal: Liver Research

Article Title: Combination of brefeldin A and tunicamycin induces apoptosis in HepG2 cells through the endoplasmic reticulum stress-activated PERK-eIF2α-ATF4-CHOP signaling pathway

doi: 10.1016/j.livres.2025.01.004

Figure Lengend Snippet: Antibodies used for Western blots.

Article Snippet: Rabbit anti-human PARP-1 , Protein Tech, China , 66520-1-IG , 1:1000.

Techniques: Western Blot

Journal: Cancers

Article Title: YY1 Knockdown Relieves the Differentiation Block and Restores Apoptosis in AML Cells

doi: 10.3390/cancers15154010

Figure Lengend Snippet: YY1 knockdown induces apoptosis in HL-60 cells. ( A ) Flow cytometry plots showing Annexin V and propidium iodide (PI) double staining in HL-60-sh-doxy (control) and sh-doxy-YY1 cells untreated and treated with 1 μM ATRA for 72 h and 96 h. ( B ) Western blots detecting PARP1, BAX, and caspase 3 protein levels in HL-60-sh-doxy (control) and HL-60-YY1 sh-YY1-doxy cells.

Article Snippet: The following primary antibodies and dilutions were used for immunoblotting detection: rabbit anti-human YY1 (1:600, sc-1703, Santa-Cruz Biotechnology; Dallas, TX, USA); rabbit anti-human PARP1 (1:4000, ALX-210-302-R100, Life Sciences); rabbit anti-human BAX (1:200, #2772, Cell Signaling Technology; Danvers, MA, USA); rabbit anti-human caspase-3 (1:800, #9662, Cell Signaling Technology); rabbit anti-human C/EBPα (1:800, EP709Y-ab40764, Abcam; Cambridge, MA, USA); rabbit anti-human C/EBPδ (1:500, EPR23518-259-ab245214, Abcam); and rabbit anti-human C/EBPε (0.6 μg/mL, ab246861, Abcam).

Techniques: Knockdown, Flow Cytometry, Double Staining, Control, Western Blot

Journal: Cancers

Article Title: YY1 Knockdown Relieves the Differentiation Block and Restores Apoptosis in AML Cells

doi: 10.3390/cancers15154010

Figure Lengend Snippet: YY1 knockdown induces apoptosis in OCI-AML3 cells. ( A ) Flow cytometry plots showing Annexin V and Live/Dead double staining in OCI-AML3-siSc-CT (control) and siYY1 cells untreated and treated with 1 μM ATRA for 96 h. ( B ) Western blots detecting PARP1 and caspase 3 protein levels in OCI-AML3 siSc-CT (control) and siYY1 cells untreated and treated with ATRA 1 μM for 96 h.

Article Snippet: The following primary antibodies and dilutions were used for immunoblotting detection: rabbit anti-human YY1 (1:600, sc-1703, Santa-Cruz Biotechnology; Dallas, TX, USA); rabbit anti-human PARP1 (1:4000, ALX-210-302-R100, Life Sciences); rabbit anti-human BAX (1:200, #2772, Cell Signaling Technology; Danvers, MA, USA); rabbit anti-human caspase-3 (1:800, #9662, Cell Signaling Technology); rabbit anti-human C/EBPα (1:800, EP709Y-ab40764, Abcam; Cambridge, MA, USA); rabbit anti-human C/EBPδ (1:500, EPR23518-259-ab245214, Abcam); and rabbit anti-human C/EBPε (0.6 μg/mL, ab246861, Abcam).

Techniques: Knockdown, Flow Cytometry, Double Staining, Control, Western Blot

Journal: Chemical Science

Article Title: Discovery of an NAD + analogue with enhanced specificity for PARP1 †

doi: 10.1039/d1sc06256e

Figure Lengend Snippet: ADO-3′-N 3 -NAD + has high substrate activity and specificity for PARP1-catalyzed protein PARylation.

Article Snippet: PARP1 expression in cell lines was examined with anti-human PARP1 monoclonal antibody (Cell Signaling Technologies: 46D11).

Techniques: Activity Assay

Journal: Chemical Science

Article Title: Discovery of an NAD + analogue with enhanced specificity for PARP1 †

doi: 10.1039/d1sc06256e

Figure Lengend Snippet: Substrate activities of NAD + analogues for protein ADP-ribosylation. (A) and (B) Substrate activities of NR-3′-N 3 -NAD + and ADO-3′-N 3 -NAD + for human PARP1 (A) and PARP2 (B). (C) Substrate activities of 2-a-NAD + and ADO-3′-N 3 -NAD + for catalytic domain of human PARP5a. (D) Substrate activities of 6-a-NAD + and ADO-3′-N 3 -NAD + for catalytic domain of human PARP10. (E) Chemical structures of NR-3′-N 3 -NAD + , 2-a-NAD + , and 6-a-NAD + . (F) Protein ADP-ribosylation for HAP1 and HAP1/PARP1-KO cell lysates with NAD + . (G) and (H) Protein ADP-ribosylation for HAP1 (G) and HAP1/PARP1-KO (H) cell lysates with NR-3′-N 3 -NAD + and ADO-3′-N 3 -NAD + . PARPs or cell lysates were incubated with NAD + or NAD + analogues in the absence or presence of olaparib at 30 °C for 2 hours for PARPs or overnight for cell lysates. The reactions with NAD + analogues were then labeled with biotin through click chemistry, followed by immunoblot analysis as detected by the streptavidin-HRP conjugate (top panels). Immunoblots for the reactions with NAD + were detected using an anti-PAR antibody. Middle panels: PARP or GAPDH loading controls as detected by an anti-His 6 antibody, anti-PARP2 antibody, or anti-GAPDH antibody. Bottom panels: densitometric analysis of protein ADP-ribosylation normalized to loading controls. ns = not significant; *, p < 0.05; **, p < 0.01.

Article Snippet: PARP1 expression in cell lines was examined with anti-human PARP1 monoclonal antibody (Cell Signaling Technologies: 46D11).

Techniques: Analogues, Incubation, Labeling, Western Blot

Journal: Chemical Science

Article Title: Discovery of an NAD + analogue with enhanced specificity for PARP1 †

doi: 10.1039/d1sc06256e

Figure Lengend Snippet: Kinetic parameters of NAD + and ADO-3′-N 3 -NAD + with human PARP1

Article Snippet: PARP1 expression in cell lines was examined with anti-human PARP1 monoclonal antibody (Cell Signaling Technologies: 46D11).

Techniques: Activity Assay

Journal: Chemical Science

Article Title: Discovery of an NAD + analogue with enhanced specificity for PARP1 †

doi: 10.1039/d1sc06256e

Figure Lengend Snippet: Potentially novel protein substrates of human PARP1 identified by ADO-3′-N 3 -NAD + . (A) A pie chart of protein substrates identified by ADO-3′-N 3 -NAD + . (B) and (C) ADP-ribosylation of HDAC2 (B) and HMGA2 (C) by human PARP1. HDAC2-His 6 or HMGA2-His 6 was incubated with NAD + in the absence or presence of His 6 -tag free PARP1 without or with veliparib at 30 °C for overnight. Reactions with only PARP1 were incubated under the same conditions. The samples were then incubated with Ni-NTA beads to separate unbound PARP1 and elute HDAC2 or HMGA2 for immunoblot analysis as detected by an anti-pan-ADP-ribose binding reagent. The total reactions were detected by the anti-pan-ADP-ribose binding reagent (top panel) or anti-His 6 antibody (bottom panels). Right panels: densitometric analysis of ADP-ribosylated proteins for eluted samples normalized to respective substrate proteins. *, p < 0.05; **, p < 0.01.

Article Snippet: PARP1 expression in cell lines was examined with anti-human PARP1 monoclonal antibody (Cell Signaling Technologies: 46D11).

Techniques: Incubation, Western Blot, Binding Assay

Journal: Frontiers in Oncology

Article Title: Chloroquine Triggers Cell Death and Inhibits PARPs in Cell Models of Aggressive Hepatoblastoma

doi: 10.3389/fonc.2020.01138

Figure Lengend Snippet: PARP1 and PARP2 are downregulated after 96 h CQ exposure in HB cells. Relative PARP1 (A) and PARP2 (B) protein expression in HUH6 (left panel), HB-284 (middle panel), and HB-243 (right panel) cells treated with 5 μM CQ detected with western blotting. Normalized band intensity of three independent sample in each group were used to calculate relative protein expression. Student's t -test was exploited for statistical analysis. * P < 0.05, ** P < 0.01. Bar plots are presented as relative values of mean ± RSD.

Article Snippet: Next, cells were incubated with primary antibody at room temperature for 1 h (#9532 human anti-rabbit PARP1 at 1:800 dilution, Cell Signaling Technologies).

Techniques: Expressing, Western Blot

Journal: Frontiers in Oncology

Article Title: Chloroquine Triggers Cell Death and Inhibits PARPs in Cell Models of Aggressive Hepatoblastoma

doi: 10.3389/fonc.2020.01138

Figure Lengend Snippet: PARP1 and PARP2 are downregulated after 96 h CQ exposure in HB cells. Relative PARP1 (A) and PARP2 (B) protein expression in HUH6 (left panel), HB-284 (middle panel), and HB-243 (right panel) cells treated with 5 μM CQ detected with western blotting. Normalized band intensity of three independent sample in each group were used to calculate relative protein expression. Student's t -test was exploited for statistical analysis. * P < 0.05, ** P < 0.01. Bar plots are presented as relative values of mean ± RSD.

Article Snippet: Membranes were incubated with following primary antibodies at +4°C for overnight: anti-human PARP1 rabbit IgG in dilution 1:1,500 (#9532; Cell Signaling Technology, Danvers, MA, USA) and anti-human PARP2 rabbit IgG in dilution 1:1,000 (#NBP2-47337; Novus Biologicals, Littleton, CO, USA).

Techniques: Expressing, Western Blot